Denaturing gradient gel electrophoresis a tool for plant protection research
نویسندگان
چکیده
منابع مشابه
Denaturing Gradient Gel Electrophoresis – a Tool for Plant Protection Research
Analysis of microbial communities associated with plants, insects and soil has been a significant challenge for plant protection researchers, because of the lack of techniques with which to access these populations. A new molecular community profiling technique, 16S rRNA gene-based PCR followed by denaturing gradient gel electrophoresis (DGGE), has been used to analyse microbial communities in ...
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We present an online tool (EquiBands, http://www.univie.ac.at/IECB/limno/equibands/EquiBands.html) that quantifies the matching of two bands considered to be the same in different samples, even when samples are applied to different denaturing gradient gel electrophoresis gels. With an environmental example we demonstrate the procedure for the classification of two bands of different samples wit...
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results the results showed that nl1-gc/ls2 primer set could yield species-specific amplicons, which were well distinguished and allowed better species discrimination than that generated by the its3-gc/its4 primer set, in both dgge and ttge profiles. all five candida species were discriminated by dgge and ttge using the nl1-gc/ls2 primer set. conclusions comparison of dgge and ttge profiles obta...
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ژورنال
عنوان ژورنال: New Zealand Plant Protection
سال: 2003
ISSN: 1179-352X,1175-9003
DOI: 10.30843/nzpp.2003.56.6056